TOTO®-three stain and one other dimeric cyanine stains have wide applicability because of their reduced background and shiny fluorescence. Works by using contain staining of nucleic acids on solid supports, prestaining of samples for gel or capillary electrophoresis, and nuclear counterstaining in many-label experiments.
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TOTO®-3 stain presents potent and selective nuclear staining in cultured cells and in paraffin sections. TOTO®-3 stain as well as other carbocyanine dimers have incredibly potent binding affinity for dsDNA, with dissociation constants inside the micromolar vary.
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